Question 1

Simple staining is often necessary to improve contrast in which microscope?

Accepted Answer

A)   fluorescence microscope
B)   phase-contrast microscope
C)   compound light microscope
D)   darkfield microscope
E)   electron microscopeF) B) and E)
G) B) and C)

Question 2

Which microscope achieves the highest magnification and greatest resolution?

Accepted Answer

A)   darkfield microscope
B)   electron microscope
C)   compound light microscope
D)   phase-contrast microscope
E)   fluorescence microscopeF) A) and E)
G) B) and C)

Question 3

Bacterial smears are fixed before staining to

Accepted Answer

A)   accept stain.
B)   affix the cells to the slide.
C)   make the cells visible.
D)   make their walls permeable.E) B) and D)
F) C) and D)

Question 4

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Accepted Answer

A) True 
 B)False

Question 5

A microorganism measures 5 μm in length. Its length in mm would be

Accepted Answer

A)   500 mm.
B)   0.5 mm.
C)   0.005 mm.
D)   0.05 mm.
E)   50 mm.F) C) and D)
G) B) and E)

Question 6

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Accepted Answer

A)   brightfield microscope
B)   phase-contrast microscope
C)   scanning electron microscope
D)   darkfield microscope
E)   transmission electron microscopeF) D) and E)
G) C) and E)

Question 7

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet
3-Safranin 4-Iodine

Accepted Answer

A)   1-3-2-4
B)   1-2-3-4
C)   2-1-4-3
D)   2-4-1-3
E)   4-3-2-1F) None of the above
G) A) and C)

Question 8

In 1884, Hans Christian Gram described a method of staining bacterial cells while not staining surrounding animal tissues. However, he thought that the staining method he developed was faulty because not all bacteria stained. In a letter to the editor of the journal in which Gram published his findings, write your response to Gramʹs concern.

Accepted Answer

The answer of In 1884, Hans Christian Gram described a...

Question 9

Which microscope can be used to visualize DNA or botulinum toxin?

Accepted Answer

A)   compound light microscope
B)   phase-contrast microscope
C)   scanning tunneling microscope
D)   scanning electron microscope
E)   confocal microscopeF) All of the above
G) C) and D)

Question 10

Which microscope uses visible light?

Accepted Answer

A)   confocal microscope
B)   differential interference contrast microscope
C)   scanning acoustic microscope
D)   scanning electron microscope
E)   fluorescence microscopeF) A) and D)
G) A) and E)

Question 11

Which of the following is NOT equal to 1 mm?

Accepted Answer

A)   100 μm
B)   10-3 m
C)   106 nm
D)   0.1 cm
E)   0.001 mF) A) and E)
G) A) and D)

Question 12

Which of the following is never useful for observing living cells?

Accepted Answer

A)   scanning electron microscope
B)   phase-contrast microscope
C)   scanning acoustic microscope
D)   brightfield microscope
E)   darkfield microscopeF) C) and D)
G) B) and E)

Question 13

In a completed Gram stain, gram-positive bacteria are purple.

Accepted Answer

A) True 
 B)False

Question 14

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

Accepted Answer

A)   an acid-fast stain
B)   a flagella stain
C)   an endospore stain
D)   a capsule stain
E)   a simple stainF) C) and E)
G) None of the above

Question 15

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point?

Accepted Answer

A)   purple
B)   red
C)   colorless
D)   brownE) A) and B)
F) A) and D)

Question 16

Which microscope uses two beams of light to produce a three-dimensional color image?

Accepted Answer

A)   phase-contrast microscope
B)   DIC microscope
C)   electron microscope
D)   fluorescence microscope
E)   darkfield microscopeF) A) and B)
G) A) and C)

Question 17

The counterstain used in the Gram stain is a basic dye.

Accepted Answer

A) True 
 B)False

Question 18

Which microscope is used to see intracellular detail in a living cell?

Accepted Answer

A)   fluorescence microscope
B)   transmission electron microscope
C)   brightfield microscope
D)   atomic force microscope
E)   two-photon microscopeF) A) and C)
G) C) and D)

Question 19

What structure does light pass through after leaving the condenser in a compound light microscope?

Accepted Answer

A)   illuminator
B)   objective lens
C)   specimen
D)   ocular lensE) None of the above
F) B) and D)

Question 20

In a completed Gram stain, gram-negative bacteria are colorless.

Accepted Answer

A) True 
 B)False

Exam 3: Observing Microorganisms Through a Microscope

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Correct Answer
verified

The counterstain used in the Gram stain is a basic dye.

Correct Answer
verified

Simple staining is often necessary to improve contrast in which microscope?

Correct Answer
verified

In a completed Gram stain, gram-positive bacteria are purple.

Correct Answer
verified

Which of the following is NOT equal to 1 mm?

Correct Answer
verified

Which of the following is never useful for observing living cells?

Correct Answer
verified

Which microscope can be used to visualize DNA or botulinum toxin?

Correct Answer
verified

A microorganism measures 5 μm in length. Its length in mm would be

Correct Answer
verified

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-Iodine

Correct Answer
verified

Which microscope uses two beams of light to produce a three-dimensional color image?

Correct Answer
verified

Bacterial smears are fixed before staining to

Correct Answer
verified

Which microscope is used to see intracellular detail in a living cell?

Correct Answer
verified

Which microscope uses visible light?

Correct Answer
verified

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

Correct Answer
verified

What structure does light pass through after leaving the condenser in a compound light microscope?

Correct Answer
verified

In a completed Gram stain, gram-negative bacteria are colorless.

Correct Answer
verified

Correct Answer
verified

Which microscope achieves the highest magnification and greatest resolution?

Correct Answer
verified

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point?

Correct Answer
verified

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Correct Answer
verified

Exam 3: Observing Microorganisms Through a Microscope

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Correct AnswerverifiedShow Answer

The counterstain used in the Gram stain is a basic dye.

Correct AnswerverifiedShow Answer

Simple staining is often necessary to improve contrast in which microscope?

Correct AnswerverifiedShow Answer

In a completed Gram stain, gram-positive bacteria are purple.

Correct AnswerverifiedShow Answer

Which of the following is NOT equal to 1 mm?

Correct AnswerverifiedShow Answer

Which of the following is never useful for observing living cells?

Correct AnswerverifiedShow Answer

Which microscope can be used to visualize DNA or botulinum toxin?

Correct AnswerverifiedShow Answer

A microorganism measures 5 μm in length. Its length in mm would be

Correct AnswerverifiedShow Answer

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-Iodine

Correct AnswerverifiedShow Answer

Which microscope uses two beams of light to produce a three-dimensional color image?

Correct AnswerverifiedShow Answer

Bacterial smears are fixed before staining to

Correct AnswerverifiedShow Answer

Which microscope is used to see intracellular detail in a living cell?

Correct AnswerverifiedShow Answer

Which microscope uses visible light?

Correct AnswerverifiedShow Answer

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

Correct AnswerverifiedShow Answer

What structure does light pass through after leaving the condenser in a compound light microscope?

Correct AnswerverifiedShow Answer

In a completed Gram stain, gram-negative bacteria are colorless.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which microscope achieves the highest magnification and greatest resolution?

Correct AnswerverifiedShow Answer

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the counterstain. What is the appearance of the bacteria at this point?

Correct AnswerverifiedShow Answer

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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