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Which of the following characteristics indicates that two organisms are closely related?


A) Their DNA can hybridize together.
B) Both are cocci.
C) Both are motile.
D) Both ferment lactose.
E) Both normally live in clams.

F) B) and E)
G) A) and B)

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Into which group would you place a photosynthetic cell that lacks a nucleus?


A) Animalia
B) Bacteria
C) Protista
D) Fungi
E) Plantae

F) B) and E)
G) None of the above

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Phage typing is based on the fact that


A) bacterial viruses attack only members of a specific species.
B) viruses cause disease.
C) bacteria are destroyed by viruses.
D) phages and bacteria are related.
E) Staphylococcus causes infections.

F) A) and B)
G) A) and C)

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Which of the following is the best evidence for a three-domain system?


A) There are three distinctly different Gram reactions.
B) There are three distinctly different sets of metabolic reactions.
C) Nucleotide sequences in ribosomal RNA vary between all three domains.
D) Some bacteria live in extreme environments.
E) There are three distinctly different types of nuclei.

F) B) and C)
G) A) and B)

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Nucleic acid hybridization is based on the fact that


A) all cells have DNA.
B) a chromosome is composed of complementary strands.
C) pairing between complementary bases occurs.
D) DNA is composed of genes.
E) the strands of DNA can be separated.

F) A) and B)
G) B) and C)

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Which of the following best defines a strain in microbiological terms?


A) a pure culture that is not totally identical to other cultures of the same species
B) groups of cells all derived from and identical to a single parent species
C) same as a species
D) a mixed population of cells,genetically distinct, but with highly similar phenotypic characteristics
E) a group of organisms with a limited geographical distribution

F) B) and E)
G) C) and E)

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Chloroplasts possess circular DNA and reproduce by binary fission.

A) True
B) False

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True

The phylogenetic classification of bacteria is based on


A) cell morphology.
B) Gram reaction.
C) habitat.
D) diseases.
E) rRNA sequences.

F) A) and B)
G) A) and C)

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Into which group would you place a unicellular organism that has 70S ribosomes and a peptidoglycan cell wall?


A) Animalia
B) Plantae
C) Bacteria
D) Fungi
E) Protist

F) A) and D)
G) B) and C)

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A biochemical test, in the microbiology world, is used to determine


A) staining characteristics.
B) amino acid sequences of enzymes.
C) capability of a microbe to perform a specific enzymatic activity.
D) nucleic acid-base composition of DNA sequences.
E) All of the answers are correct.

F) B) and C)
G) All of the above

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C

A clone is


A) a genetically engineered cell.
B) a mound of cells on an agar medium.
C) a taxon composed of species.
D) genetically identical cells derived from a single cell.
E) None of the answers is correct.

F) D) and E)
G) B) and C)

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Figure 10.2 A nucleic acid hybridization experiment produced the following results. Figure 10.2 A nucleic acid hybridization experiment produced the following results.   In Figure 10.2, which figure shows the most closely related organisms? A)  a B)  b C)  c D)  d E)  e In Figure 10.2, which figure shows the most closely related organisms?


A) a
B) b
C) c
D) d
E) e

F) B) and E)
G) None of the above

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The arrangement of organisms into taxa


A) shows relationships to common ancestors.
B) shows degrees of relatedness between organisms.
C) is arbitrary.
D) is based on evolution.
E) was designed by Charles Darwin.

F) A) and B)
G) B) and C)

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All of the following statements are reasons why fluorescent in situ hybridization (FISH) has become a valuable tool for environmental microbiologists EXCEPT


A) it allows observation of microbes in their natural environment in association with other microbes.
B) it allows one to obtain pure cultures of microbes.
C) it demonstrates the diversity of microbes in an environment.
D) it allows for detection of uncultured microbes.
E) All of the answers are correct.

F) A) and B)
G) C) and D)

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If two organisms have similar rRNA sequences, you can conclude that they


A) live in the same place.
B) will have different G-C ratios.
C) mated with each other.
D) will both ferment lactose.
E) evolved from a common ancestor.

F) None of the above
G) A) and B)

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Serological testing is used for screening bacterial isolates for similarities.

A) True
B) False

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The highest level in the current taxonomic hierarchy is ʺKingdom.ʺ

A) True
B) False

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Serological testing is based on the fact that


A) the human body makes antibodies against bacteria.
B) antibodies react specifically with an antigen.
C) bacteria clump together when mixed with any antibodies.
D) antibodies cause the formation of antigens.
E) all bacteria have the same antigens.

F) None of the above
G) D) and E)

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Table 10.2 I. Gram-positive A. Catalase ++ 1. Acid from glucose........... Staphylococcus 2. Glucose.........................Micrococcus B. Catalase- 1. Coccus.........................Streptococcus 2. Rod ..........................Lactobacillus II. Gram-negative A. Oxidase- 1. Acid from lactose a. Uses citric acid..........................Citrobacter b. Citric acid.................................Escherichia 2. Lactose- a. H2 S\mathrm { H } _ { 2 } \mathrm {~S} produced (1) Urease positive............................Proteus (2) Urease negative.............................. Salmonella B. Oxidase ++ 1. Rod...........................Pseudomonas 2. Coccus....................... Neisseria -Use the dichotomous key in Table 10.2 to identify a gram -negative coccus.


A) Micrococcus
B) Neisseria
C) Pseudomonas
D) Staphylococcus
E) Streptococcus

F) A) and E)
G) A) and B)

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Which of the following statements is a reason for NOT classifying viruses in one of the three domains of life (Bacteria, Archaea, Eukarya) rather than in a fourth separate domain?


A) Viruses direct anabolic pathways of host cells.
B) Viruses are not composed of cells.
C) Some viruses can incorporate their genome into a hostʹs genome.
D) Viruses are obligate parasites.
E) All of the answers are correct.

F) A) and B)
G) B) and C)

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B

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